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What are Branched Amphipathic Peptide Capsules (BAPC™)?

BAPC are an entirely new class of nanocarrier engineered from two naturally occurring peptide sequences found in a human heart channel protein. They are a superior delivery vehicle for proteins, peptides and nucleic acids, since they overcome many of the poor absorption issues that plague current nanodelivery products. BAPC have been demonstrated to substantially improve the efficacy and delivery of novel vaccines, cancer therapies, diagnostics and biopesticides. The surface of BAPC allows for attachment of targeting moieties, ensuring active ingredients are delivered precisely to the cells where they are needed.

A Fully Customizable Carrier

Can encapsulate water soluble compounds

Programmable in size from 6-2,000 nm

Surface binding capacity

BAPC Advantages

Water soluble

Non-immunogenic

Extremely stablecan withstand temperatures up to 100° C

Minimally toxic to cells

Biodegradable

A nanocarrier that overcomes the
limitations of existing products

Product Compound Cytotoxic Immune Response Stability Biodegradable Company
BAPtofect™-25 Compound:BAPC Cytotoxic:Extremely Low Response:No Stability:High Biodegradable:Yes Company:Phoreus
Lipofectamine 2000 Compound:Lipid Cytotoxic:Moderate Response:Yes Stability:Low Biodegradable:Yes Company:Thermo Fisher
X-tremeGene Compound:Polymer Cytotoxic:Low Response:No Stability:High Biodegradable:No Company:Roche
HiPerFect Compound:Lipid Cytotoxic:Moderate Response:Yes Stability:Low Biodegradable:Yes Company:Quigen
baculoFECTIN Compound:Polymer Cytotoxic:Low Response:No Stability:High Biodegradable:No Company:Oxford
Trans-Hi Compound:Polymer Cytotoxic:Low Response:No Stability:High Biodegradable:No Company:FormuMax
TransITxX2 Compound:Polymer Cytotoxic:Low Response:No Stability:High Biodegradable:No Company:Mirus Bio

How does it Work?
Here are the steps for BAPC Payload Delivery:

1

The positively charged surface of BAPC meets a cell membrane.

2

The cell membrane envelopes BAPC and their cargo, forming an early endosome.

3

Unable to breakdown the BAPC, the late endosome wall breaks down releasing the contents in the perinuclear cytosol.

4

The new pH results in a reduction of electrostatic attraction, and the payload is released.

5

Nucleic acids are released from the BAPC into the cytosol.

Applications for In Vitro
and In Vivo Research

Encapsulation or Surface Binding Capacity


Encapsulation of Water-Soluble Compounds: Fluorescent dyes, radionuclides, colloidal gold, magnetic beads

Surface Binding Capacity: Proteins, peptides, nucleic acids, oligonucleotides, plasmids and CRISPR-Cas9 components

BAPC are rapidly taken up by all cells tested to-date, including a variety of epithelial cells, macrophage and adipocytes. They enter cells through the endocytic pathway and escape the late endosomes, finally accumulating in the perinuclear space. BAPC have been shown to effectively deliver nucleic acids (DNA and RNA), which are released in a time-dependent manner. They show negligible cytotoxicity when given at doses that exceed clinically relevant ones in cultured cells, fungi, insects, nematodes, plants and mice. Working with BAPC makes it simple to move from in vitro research to in vivo applications.