Development of Novel Chimeric Vaccine and Delivery System for Classical Swine Fever Virus
ABSTRACT:To eliminate the need for a cold chain, DNA vaccine approach was used, in which nanoparticles composed of branched amphiphilic peptide capsules (BAPC) were employed as the DNA delivery agent. The candidate vaccine and the DNA delivery system were evaluated in in vitro expression system and a nursery pig model.…
Continue ReadingGermline Mutagenesis of Nasonia Vitripennis Through Ovarian Delivery of CRISPR-Cas9 Ribonucleoprotein
ABSTRACT:CRISPR/Cas9 gene editing is a powerful technology to study the genetics of rising model organisms, such as the jewel wasp Nasonia vitripennis. However, current methods involving embryonic microinjection of CRISPR reagents are challenging. Delivery of Cas9 ribonucleoprotein into female ovaries is an alternative that has only been explored in a small…
Continue ReadingBiodegradable Drug-Delivery Peptide Nanocapsules
ABSTRACT:Branched amphiphilic peptide capsules (BAPCs) are an efficient transport system that can deliver nucleic acids, small proteins, and solutes. The ability of BAPCs to break down is essential to their adoption as a delivery vehicle for human and agricultural applications. Until now, however, BAPCs were shown to be inert to…
Continue ReadingBranched Amphiphilic Peptide Capsule Uptake by Aspergillus Nidulans
ABSTRACT:The ability of branched amphipathic peptide capsules (BAPC) to encapsulate and transport payloads into cells offers new approaches to deliver active ingredients (AIs). Until now, we have found that the BAPC were completely inert in previously tested eukaryotic cells. However, photographs have been collected that demonstrate Aspergillus nidulans, a common…
Continue ReadingTransdermal Delivery of Branched Amphipathic Peptide Capsule Magnetic (BAPC-MB) in Mice
ABSTRACT:To test the transdermal delivery capabilities of BAPC-MB, the tails of live mice were dipped in BAPC-MB solution for 1, 5, 15 and 30 minutes. After a 24-hour incubation period, the tissues were harvested for evaluation of tissue distribution. This initial proof-of-concept experiment demonstrates that 50 nm BAPC-MB are rapidly…
Continue ReadingRat Intestinal Epithelial Cells (IEC-18) Internalize Magnetic Nanobeads (MNBs) Encapsulated Within a Branched Amphipathic Peptide (BAP) Bilayer
ABSTRACT:To evaluate the effect of BAP encapsulation of magnetic nanobeads on both cellular uptake and cell viability, research was conducted with Branched Amphipathic Peptide Capsule Magnetic Beads (BAPC-MB) and rat intestinal epithelial cells (IEC-18). The study found that the percentage of IEC-18 cells taking up BAPC-MB was significantly higher when…
Continue ReadingPhysical Characteristics of 50 and 200 nm Magnetic Beads when Encapsulated with Branched Amphiphilic Peptides (BAP)
ABSTRACT:Branched Amphipathic Peptide Capsule Magnetic Beads (BAPC-MB) are a new nanoparticle developed for use as in diagnostic platforms. The bases are Maleimide Super Mag Magnetic Beads which are dispersed in 75% ethanol and combined with our proprietary H9Cys BAP. The result of the initial step is a magnetic bead with…
Continue ReadingTwo Separate Toxicity Studies Conducted on Apis Melifera (Honey Bees)
ABSTRACT:Introduction This report outlines two separate toxicity studies conducted on Apis melifera. The first study follows the parameters of the EPA Litmus test and the second study delves deeper into possible BAPC toxicity surpassing the standards of the EPA definitive test. Each study was performed under EPA guidelines including harvesting…
Continue Reading